There is no way how MKV multimedia files could be converted to ASS subtitles. However, you can simply export subtitles from existing MKV files and save them in one of the suitable formats like the ASS.
Home > Search converters: mkv to ass
Conversion of mkv file format to ass file format beta
Search for mkv to ass converter or software able to handle these file types.
Convert Matroska multimedia container to Advanced SubStation Alpha subtitles.
We have found 4 software records in our database eligible for .mkv to .ass file format conversion.
Microsoft Windows software 
- convert mkv to ass on Windows
MKVExtractGUI-2
A Matroska extraction utility
File extension | Convert | Open | Save | Edit | Create | Import | Export | Extract |
|---|---|---|---|---|---|---|---|---|
Convert from mkv file | No | No | No | No | No | Yes | No | No |
to ass file | No | No | No | No | No | No | Yes | No |
The tables with software actions are a good pointer for what a certain program does with various file types and often may give users a good hint how to perform certain file conversion, for example the above-mentioned mkv to ass. However, it is not perfect and sometimes can show results which are not really usable because of how certain programs work with files and the possible conversion is thus actually not possible at all.
MKVtoolnix
A program for working with Matroska MKV files
Apple macOS / Mac OS X software 
- convert mkv to ass on OS X
MKVtoolnix for Mac
A Mac version of the video editing suite for Matroska
Linux/Unix software 
- convert mkv to ass on Linux
MKVtoolnix for Linux
A set of utilities for handling Matroska files in version for Linux
Additional links: mkv file extension details, ass file extension details
MKV
An mkv file extension file extension is used for Matroska Multimedia Container format, which is an open source video format very often used for HD movies. You can open it in almost any media player as the format is largely supported.
ASS
Files with ass extension are in most cases movie (video) subtitles extension saved in Aegisub Advanced Substation. Playable in most media players nowadays.
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