There is no way how MKV multimedia files could be converted to ASS subtitles. However, you can simply export subtitles from existing MKV files and save them in one of the suitable formats like the ASS.
Home > Search converters: mkv to ass
Conversion of mkv file format to ass file format beta
Search for mkv to ass converter or software able to handle these file types.
Convert Matroska multimedia container to Advanced SubStation Alpha subtitles.
We have found 4 software records in our database eligible for .mkv to .ass file format conversion.
Microsoft Windows software 
- convert mkv to ass on Windows
MKVExtractGUI-2
Matroska (mkv) track extraction windows GUI tool for work with mkvtoolnix.
File extension | Convert | Open | Save | Edit | Create | Import | Export | Extract |
|---|---|---|---|---|---|---|---|---|
Convert from mkv file | No | No | No | No | No | Yes | No | No |
to ass file | No | No | No | No | No | No | Yes | No |
The tables with software actions are a good pointer for what a certain program does with various file types and often may give users a good hint how to perform certain file conversion, for example the above-mentioned mkv to ass. However, it is not perfect and sometimes can show results which are not really usable because of how certain programs work with files and the possible conversion is thus actually not possible at all.
MKVtoolnix
A a set of tools to create, alter, split, join and inspect Matroska files (.mkv), MKVtoolnix offers users everything they need for handling Matroska format.
Apple macOS / Mac OS X software 
- convert mkv to ass on OS X
MKVtoolnix for Mac
MKVtoolnix for Mac is a video editing suite that allows users to create, alter, split, join and inspect Matroska files (mkv).
Linux/Unix software 
- convert mkv to ass on Linux
MKVtoolnix for Linux
MKVtoolnix allows users to create, alter, split, join and inspect Matroska files (.mkv, .mk3d etc.)
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